Coding

Part:BBa_K1539000:Design

Designed by: Coleen Tran   Group: iGEM14_GeorgiaTech   (2014-10-08)


HP_HR_mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When adding promoter, the EcoRI site was not maintained in the PCR product/insert, but when ligated to backbone cut with XbaI and PstI, EcoRI is retained.


Source

BBa_J06504

References